primary rabbit polyclonal antibodies against rat cd133  (Boster Bio)

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response

doi: 10.1631/jzus.b1600500

Figure Lengend Snippet: Fig. 2 Representative photomicrographs of immunohistochemical staining Adjacent sections of lung tissues showing the expressions of CD133, VEGFR-2 as well as HGF in a normal pulmonary arteriole (a‒c), an immature plexiform lesion (PL) (d‒f), and a mature lesion (g‒i). The lung sections presented here are from 2-week-old broilers. Mɸ: foam-like macrophage. Scale bar=25 μm

Article Snippet: After blocking with 5% (0.05 g/ml) bovine serum albumin, the slides were incubated with the following antibodies: polyclone rabbit anti-rat CD133 (1:50 (v/v), Bostar Biological Technology, China), VEGFR-2 (1:50 (v/v), Boster Biotechnology Technology, China), and HGF (1:50 (v/v), Bostar Biological Technology, China).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response

doi: 10.1631/jzus.b1600500

Figure Lengend Snippet: Fig. 3 Characterization of endothelial progenitor cells (EPCs) EPCs displayed a spindle-shape appearance on Day 7 of plating (a) and differentiated into monolayer of cobblestone-like cells on Day 14 (b). The ability of EPCs for DiL-ac-LDL uptake (red color) and the binding of FITC-UEA-1 lectin (green color) are shown in (c), while cells carrying both characteristics appear in orange. (d) Expressions of CD133 and VEGFR-2 were assessed by flow cytometry (gray line indicates the isotype control; black line indicates the antibody staining). Representative results are shown. Scale bar= 100 μm (Note: for interpretation of the references to color in this figure legend, the reader is referred to the web version of this article)

Article Snippet: After blocking with 5% (0.05 g/ml) bovine serum albumin, the slides were incubated with the following antibodies: polyclone rabbit anti-rat CD133 (1:50 (v/v), Bostar Biological Technology, China), VEGFR-2 (1:50 (v/v), Boster Biotechnology Technology, China), and HGF (1:50 (v/v), Bostar Biological Technology, China).

Techniques: Binding Assay, Flow Cytometry, Control, Staining